By Alexander Nakeff (auth.), Frederick A. Valeriote Ph.D., Alexander Nakeff Ph.D., Manuel Valdivieso M.D. (eds.)
The concentration of this symposium used to be at the current and destiny features of move cytometry for either scientific and organic purposes in melanoma. This expertise started with really modest instrumentation, with constrained features to respond to organic questions. this present day, either the medical workhorses and the robust multi-laser, multi-detector, sorting equipment, coupled with subtle pcs and garage units and the expanding storehouse of markers and dyes, are taking us to the restrict and past find solutions to the reason and healing of melanoma.
some time past, either basic hematopoietic tissue and leukemias were the tissue samples of selection within the software of move cytometry, and a few of the newest purposes with those tissues are offered right here. besides the fact that, the ebook additionally discusses the more and more refined disaggregation ideas which permit investigators the chance to coach their lasers on strong tumors. not just do we use stream cytometry with linked fluorescent markers to appreciate the biology of melanoma, but additionally the big choice of current and constructing markers presents us with vital diagnostic instruments within the detection of melanoma early in both the malignant or relapse procedure. And the sector comes complete circle, with using the know-how for gene mapping and different genetic experiences to unencumber the elemental malignant process.
Read or Download Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992 PDF
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Additional info for Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992
However, a major reason for drug resistance resides at the cellular level and often involves cellular mechanisms which under normal conditions may-have other protective and important biological roles. Tumor cell resistance is believed to be multifactorial involving altered drug transport (influx, retention and efflux), and biochemical mechanisms such as xenobiotic detoxification, alternate metabolic pathways, and altered targets (1-3). Multiple drug resistance (MDR) has been recently described as a phenomenon in which tumor cells are resistant to a variety of unrelated natural products such as alkaloids and antibiotics used as cancer chemotherapeutic agents (1,3).
Motility was significantly ele- vated over that of vehicle-treated controls for 6-16 h after the end of the PMA pulse; after this point it declined to control levels (Table 1). Analysis of the cell-cycle profiles of total populations and motile fractions was generally performed 4-6 h after the PMA pulse. Cell-Cycle Analysis Fresh, unstimulated lymph node lymphocytes from untreated animals can be assumed to be almost exclusively in GO' They were therefore used as a reference popu- 39 Table 1 Development and Decline of PMA-Induced Motility in Murine T Lymphocytes.
Mathematical aggregate compensation produces significant modifications of S-phase estimates which have been shown to correlate with microscopic assessments of aggregates (16). Utility in clinical follow-up series, however, has yet to be fully established. 26 Multiparametric Analysis Optimization of cell cycle calculations discussed until this point have been largely cosmetic. They have not addressed the real issue - presence of overlapping cell cycle distributions from different, biologically distinct, populations.
Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992 by Alexander Nakeff (auth.), Frederick A. Valeriote Ph.D., Alexander Nakeff Ph.D., Manuel Valdivieso M.D. (eds.)
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